TECHNIQUE FOR RAPID GROWTH OF VIBRIO FETUS IN BROTH
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Enhanced broth media for selective growth of Vibrio vulnificus.
Rapid detection of Vibrio vulnificus can be enhanced by optimizing the components of enrichment broth. PNC (5% peptone, 1% NaCl, and 0.08% cellobiose [pH 8.0]) enhanced the growth of V. vulnificus compared to alkaline peptone broth. PNCC (PNC with 1.0 to 4.1 U of colistin methanesulfonate per ml) increased the growth of low levels of V. vulnificus while suppressing non-target bacteria.
متن کاملA multipathogen selective enrichment broth for simultaneous growth of Salmonella spp., Vibrio parahaemolyticus, and Vibrio cholerae.
A selective enrichment broth (SVV) was formulated to allow concurrent growth of Salmonella spp., V. parahaemolyticus, and V. cholerae. Potassium tellurite and sodium citrate were added as the inhibitors, while glucose, mannitol, anhydrous sodium sulfite and sodium pyruvate were employed as the growth-promoters. When mixed in equal or varied proportions, the target pathogens in SVV had a great a...
متن کاملNutrition of Vibrio fetus.
Smibert, Robert M. (Virginia Polytechnic Institute, Blacksburg, Va.). Nutrition of Vibrio fetus. J. Bacteriol. 85:394-398. 1963.-A chemically defined medium has been developed for the cultivation of both catalase-positive H(2)S-negative and catalase-positive H(2)S-positive strains of Vibrio fetus. A total of 87 strains of V. fetus, representing bovine, ovine, porcine, human, and chicken isolate...
متن کاملAgglutinin-adsorption analysis of Vibrio fetus.
Reich, C. V. (Pennsylvania State University, University Park), C. E. Heist, and H. W. Dunne. Agglutinin-adsorption analysis of Vibrio fetus. J. Bacteriol. 82:210-214. 1961.-All pathogenic strains of Vibrio fetus are apparently members of a single serotype. Within this broad grouping, it was possible to define the relationship of certain selected strains by a system of complete reciprocal adsorp...
متن کاملRapid urea broth test for yeasts.
A rapid, miniaturized, urea broth test useful for detecting urease activity of yeasts was compared to Christensen urea agar. All urease-producing yeasts tested were positive on both media; however, 60% were reactive in the urea R broth within 30 min, and the remainder were reactive within 4 h. This urea multiwell test may be useful as a rapid screening method for detecting urease-producing yeas...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1957
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.74.2.246-250.1957